Banca de DEFESA: FLAVIA GEOVANA FONTINELES RIOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : FLAVIA GEOVANA FONTINELES RIOS
DATA : 30/03/2020
HORA: 14:00
LOCAL: Programa de Pós Graduação em Biologia
TÍTULO:

DETECTION OF ARBOVIRUS IN MOSQUITOES (DIPTERA: CULICIDAE) IN THE URBAN AREA OF PORTO VELHO, RONDÔNIA.

PALAVRAS-CHAVES:

DENV-4. Infection rate. Vertical transmission. ZIKV.

PÁGINAS: 90
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
RESUMO:

Os mosquitoes have a worldwide distribution, being present in urban, rural and forest environments. Urbanization without planning favors the proliferation and survival of mosquito vectors and diseases in cities. In Brazil, the species of urban mosquitoes that stand out are Aedes aegypti, Aedes albopictus and Culex quinquefasciatus. The three species are associated with the transmission of several arboviruses, such as the Dengue, Zika and Chikungunya and Wuchereria bancrofti viruses (etiological agent of lymphatic filariasis) for the latter species. The general objective of the present work was to verify the abundance of the three main species of mosquitoes and to evaluate the possible occurrence of arboviruses in their populations, in the urban area of Porto Velho, Rondônia. Initially, a preliminary study was carried out in the Tancredo Neves neighborhood with household collections, with the purpose of adapting field and laboratory techniques, and subsequently a study was carried out in 27 residences distributed in nine neighborhoods in intra and peridomestic environments. Two collection techniques were used: ovitrampa (without reed, only for larvae collection) and electric aspiration for the capture of adults lasting 15 minutes. The adults, generated in the laboratory from the larvae, were used to verify the vertical transmission of DENV, CHIKV and ZIKV. The specimens captured by the electric aspiration technique had, in addition to the body, saliva collected on FTA® cards for analysis of the presence of these viruses. The samples were submitted to the RNA extraction process, followed by RT-qPCR in real time. The positive pools were also submitted to a semi-nested PCR protocol and subsequently to capillary sequencing. The species abundance data were analyzed in the Free Platform R and the estimate of infection by virus and by species was calculated by the Minimum Infection Rate (MIR) and Maximum Likelihood (MLE). In the preliminary study, 1,701 mosquitoes were collected, of which 1,332 were used for virus detection, resulting in 76 pools divided between: Culex quinquefasciatus (57), Aedes aegypti (18) and Aedes spp. (1), obtaining two positive pools for ZIKV, resulting in an MIR of 1.50 and 1.51 of MLE. Subsequently, a total of 2,342 mosquitoes were obtained in the nine sampled neighborhoods, distributed in five species, being Cx. Quinquefasciatus, Ae. aegypti and Ae. albopictus the most abundant, constituting 272 pools of which one was positive for DENV (MIR and MLE: 2.58). In addition, it was possible to detect DENV-4 from a sample of saliva collected on an FTA card, which belonged to a female of the Ae species. aegypti. The sequences of the positive samples were identified with DENV-4 genotype II. This study demonstrates the first record of DENV and ZIKV transmission in urban mosquitoes in Rondônia, as well as showing the first occurrence of vertical / venereal transmission of DENV-4 and ZIKV in male mosquitoes of the species Ae. aegypti and Cx. quinquefasciatus, respectively.

MEMBROS DA BANCA:
Presidente - 902.446.996-15 - GENIMAR REBOUÇAS JULIÃO - UNIR
Externo à Instituição - MICHELLE DE OLIVEIRA E SILVA - FIOCRUZ
Interno - 582.482.422-34 - NAJLA BENEVIDES MATOS - UNIR