Fauna, molecular detection of Leishmania (Kinetoplastida: Trypanosomatidae) and food preference of sandflies (Diptera: Psychodidae) in districts from the municipality of Porto Velho, Rondônia, Brazil
Keywords: Phlebotomines. Leishmania. Diversity. Natural infection. Sources of repast.
Sandflies are small insects that feed on plant sap, only females perform hematophagy. This habit allows ingestion and transmission of pathogens, including protozoa of the genus Leishmania. Soon, Some species are of medical importance. In Rondônia, already 135 species of sandflies were recorded, including vectors and species with transmission potential. Therefore, the purpose of this paper is to understand aspects diversity, natural infection and food source of sand flies in the municipality of Porto Velho. The study was conducted in the following districts of Porto Old Man: Nova Mutum Paraná, Bandeirantes Union, Vista Alegre do Abunã and Nova California. Sand flies are being collected using light traps HP, exposed in the forest, edge and peridomicile ecotopes. After screening and taxonomic identification, the thorax and abdomen of engorged females and females are for DNA extraction and PCR directed to the kDNA, hsp70 and Cytb genes. To date, 1,335 specimens have been identified, distributed in 51 species and 14 genres. Among the most abundant species, Psychodopygus davisi, Trichophoromyia auraensis, Nyssomyia antunesi, Psychodopygus hirsutus hirsutus, Trichophoromyia ubiquitalis, Psychodopygus carrerai carrerai, and Psychodopygus complexus have already been incriminated or are considered potential vectors. Until the At this time, the district of New California had the largest abundance (1,033 individuals) and wealth (36 species). Forest ecotopes presented the largest abundance (1,133 individuals) and wealth (46 species). In the peridomiciliaries, even With low abundance (39 individuals), the richness was 15 species. This corroborates with previous studies demonstrating the abundance of these insects primarily forest environments and gradual adaptation to anthropized or suffered some human interference. The presence of Ps. davisi, Th. auraensis, Ny. antunesi and Th. ubiquitalis in the three ecotopes indicate the adaptation of these insects to different habitats. These species are considered vectors or potential vectors, depending on the region of the country in which they are found and can therefore act in Leishmania transmission in the region.