Banca de DEFESA: ENMANUELLA HELGA RATIER TERCEIRO DE MEDEIROS
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : ENMANUELLA HELGA RATIER TERCEIRO DE MEDEIROS
DATA : 23/06/2020
HORA: 08:30
LOCAL: Programa de Pós Graduação em Biologia Experimental
TÍTULO:
Keywords: L. braziliensis. Susceptibility. LRV1.
PALAVRAS-CHAVES:
Keywords: L. braziliensis. Susceptibility. LRV1.
PÁGINAS: 90
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
RESUMO:
Leishmaniasis presents itself as an endemic of non-contagious infectious characteristics and of anthropozoonotic character. There are about 350 million people exposed to the risk of contracting this infection and 2 million new cases of this endemic disease are registered annually, with a an estimated 20,000 to 30,000,000 deaths per year worldwide. Treatment of patients with cutaneous leishmaniasis in most countries is performed with the antimonial drug pentavalent (Sb + 5), which may be meglumine antimoniate (Glucantime) or stibogluconate sodium (Pentostam). However, it has been observed in recent years that some strains of Leishmania spp. have less sensitivity to Sb + 5 compounds. One of the possible factors associated with lower sensitivity in the Americas is the presence of the endosymbiont “Leishmania Virus RNA ”type 1 (LRV1) in some strains of Leishmania spp. Faced with the problematic presented, the present work seeks to analyze the groups LRV1 positive and LRV1 negative with in vitro susceptibility to the trivalent antimonial - emetic tartar (Sb + 3), seen in some studies that Sb + 5 is a prodrug and through biotransformation it becomes Sb + 3, form active and toxic antimony. For this, 20 strains of Leishmania braziliensis were selected isolated from patients treated at the Center for Tropical Medicine of Rondônia (CEMETRON) diagnosed with LT, 10 of which harbor the LRV1 symbiote. So far, In vitro tests were carried out with Sb + 3 of 15 strains of L. braziliensis, with 8 LRV1 positive and 7 LRV1 negative. RNA was also extracted from these strains for later quantification of viral load of positive strains for LRV1 and analysis of gene expression related to redox metabolism. The results obtained in the in vitro experiments showed two groups: (i) susceptible and (ii) less susceptible. Of the 15 strains analyzed, 12 presented profiles susceptible and 3 less susceptible. In addition, qPCR was standardized for quantify viral load, as well as analyze Leishmania genes involved in metabolism redox. The results obtained in in vitro susceptibility to the compound Sb + 3 will be correlated with the presence of the endosymbiont LRV1, viral load and gene expression involved in Leishmania redox metabolism. Given the above, it is known that it is extremely understanding the in vitro susceptibility of strains isolated from LT patients because these findings can provide answers of great relevance for the monitoring and treatment of LT and parasitic infection harboring endosymbiont LRV1.
MEMBROS DA BANCA:
Presidente - 087.927.277-51 - GABRIEL EDUARDO MELIM FERREIRA - UNIR
Externo à Instituição - MARIANA CORTES BOITÉ - FIOCRUZ
Externo à Instituição - SORAYA DOS SANTOS PEREIRA - FIOCRUZ