Banca de DEFESA: GLAUCILENE DA SILVA COSTA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : GLAUCILENE DA SILVA COSTA
DATA : 16/12/2021
HORA: 08:30
LOCAL: Sala Virtual Remota
TÍTULO:

Diversidade Genética e Taxonomia Integrada de Flebotomíneos (Diptera: Psychodidae) Amazônicos

PALAVRAS-CHAVES:

Amazon. DNA Barcoding. Leishmania. Rondônia. Vectors.

PÁGINAS: 96
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Sand flies are important insects in public health that act as vectors of Leishmania, that cause leishmaniasis. In Rondônia State, studies about sand flies have been carried out since the 1960s which knowledge of local fauna is important for identification of vectors and understanding of the dynamics of disease transmission. Currently, sand fly identification is based on morphology of adults, which can be problematic when species are morphologically similar. The diversity of these insects and occurrence of cryptic species in Rondônia makes it difficult the identification based only on morphological characters, then approaches that help the classical taxonomy can improve the accuracy of the correct species identification. Therefore, this study aimed to implement the use of DNA Barcoding in Amazonian sand flies to optimize the species identification and investigate the existence of cryptic species, associating this approach to classical taxonomy in the description of fauna of the region. For this study, specimens from other projects carried out by Fiocruz Entomology Laboratory were used, in addition to additional collections carried out in four municipalities of Rondônia. A toral of 652 specimens belonging to 69 morphospecies were morphologically identified, then legs or thorax were removed, and their genomic DNA were extracted, and the cytochrome c oxidase subunit I (coI) fragment was amplified and sequenced. Sequences were analyzed by best-match and best close match similarity criteria and six different delimitation methods of single-locus (ABGD, RESL, TCS, GMYC, PTP and mPTP) were used to infer operational taxonomic units (OTUs). Sequences data of coI region produced in this study was evaluated with those previously published, and the database was analyzed using ABGD and TCS species delimitation methods. The gene coI proved to be useful for identification of approximately 80% of analyzed species from Rondônia, not being effective in separation in some species of the genus Evandromyia, Nyssomyia, Trichophoromyia and Trichopygomyia. The analyzes showed the presence of cryptic diversity, in at least four analyzed algorithms, for the species: Bichromomyia flaviscutellata, Evandromyia bacula, Evandromyia tarapacaensis, Evandromyia wilsoni, Nyssomyia fraihai, Psychodopygus carrerai, Psychodopygus davisi and Trichophoromyia ubiquitalis. Also was possible to establish the association between males and their respective females, which have isomorphic process between species. The approach also enabled description of Pintomyia fiocruzi and Psathyromyia pradobarrientosi females. Sequence analyzes of specimens from other locations and Rondônia demonstrate the importance of using a robust database to improve accuracy in delimitation of sand fly species. When barcodes of closely related species or from different geographic regions were added, changes were observed in delimitation of some species, showing events such as a much greater cryptic diversity or grouping closely related species. The results presented reinforce the need for molecular identification as a complement to morphological identification, demonstrating the importance of using this approach to reveal the cryptic diversity of some sand fly species.

MEMBROS DA BANCA:
Externo à Instituição - ANDREA FERNANDES BRILHANTE - UFAC
Externo à Instituição - EUNICE GALATI - USP
Interno - 087.927.277-51 - GABRIEL EDUARDO MELIM FERREIRA - UNIR
Presidente - 838.495.134-91 - JANSEN FERNANDES MEDEIROS - UNIR
Externo à Instituição - PALOMA HELENA FERNANDES SHIMABUKURA - FIOCRUZ