Banca de QUALIFICAÇÃO: MARCOS EDUARDO PASSOS DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MARCOS EDUARDO PASSOS DA SILVA
DATE: 07/07/2023
TIME: 08:00
LOCAL: Sala Virtual Remora
TITLE:

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KEY WORDS:

Pseudomonas aeruginosa. Carbapenems. Biofilm. Multiresistance

PAGES: 99
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Pseudomonas aeruginosa is among the main microorganisms causing Healthcare-Associated Infections (HAIs), standing out as an opportunistic pathogen in Intensive Care Units (ICU). The great urgency surrounding this species is due to the increasing number of strains resistant to the various antibiotics used in its antibiotic therapy, including carbepenems, which among conventional antibiotics are the main choice for the treatment of infections. In these terms, multiple resistance mechanisms are associated with P. aeruginosa, such as expression of genes encoding enzymes that inactivate or hydrolyze the antimicrobials, and virulence factors such as biofilm. Therefore, the aim of this study was to molecularly identify the mechanisms of resistance in isolates of P. aeruginosa from patients hospitalized and hospital environment of ICUs in Porto Velho/RO. The collections were performed between the years 2017 and 2018 in three public reference hospitals. 216 isolates were obtained, of which 162 were from patients: Oral cavity (78); Tracheostomy (37); Axilla (24); Urine (11); Blood (6); Wound secretion (6). There were 54 hospital structures: Sink (22); Faucet (19); Bed (11) and Floor (2). As for the resistance profile, the highest rate of non-susceptibility was to Imipenem with 33.7% (73/216) and Meropenem with 29.6% (64/216). Of susceptible isolates, 89.3% (193/216) were susceptible to Piperacycline-Tazobactam, 83.7% to Ceftolozane-Tazobactam and 82.8% to Ceftazidime-Avibactam. The multidrug resistance (MDR) phenotype was presented by 30% (65/216) of the isolates, of which 84.6% (55/65) were from patients. Of the MDR strains, 3.1% (2/65) were not susceptible to Polymyxin B and 92.3% (60/65) were moderate/strong biofilm producers. As for resistance genes, the highest prevalence was blaCTX-M with 38.8% (84/216), followed by blaTEM with 29.1% (63/216), 4.1% (9/216) of blaSHV, and 0.4% (1/216) of blaIMP and blaKPC. Furthermore, we observed the presence of P. aeruginosa clones in different sites of the same patient, as well as in hospital structures and patients, and in different hospitals carrying resistance genes. These data serve as an alert for the risk of dissemination of this pathogen harboring resistance genes that may impact on HAIs and public health problems.

COMMITTEE MEMBERS:
Externa à Instituição - SUELY CARLOS FERREIRA SAMPAIO - UNIFESP
Externo à Instituição - IVSON CASSIANO DE OLIVEIRA SANTOS
Presidente - 2148421 - CHRISTIAN COLLINS KUEHN