Banca de QUALIFICAÇÃO: THIAGO REIXACH PIRES DE SOUZA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : THIAGO REIXACH PIRES DE SOUZA
DATE: 10/10/2023
TIME: 14:00
LOCAL: Sala Virtual Remora https://bit.ly/QualifMSc_ThiagoSouza_PGBIOEXP
TITLE:

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KEY WORDS:

Acinetobacter baumannii, hybrid model, Phyllomedusa camba and virtual screening.

PAGES: 172
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

 

Antibiotics are naturally occurring or synthetic antimicrobials used to treat bacteria by bacteria. However, the inappropriate and indiscriminate use of antibiotics has favored the emergence of strains of bacteria that are multiresistant to these drugs, which has become a serious public health problem. One of the main species of bacteria that cause infections in the hospital environment are the multidrug-resistant strains of Acinetobacter baumannii. The World Health Organization points to Acinetobacter baumannii as a priority in the development of new antibiotics, as these maintain resistance control that allows escaping the most effective classes of antibiotics. Therefore, we propose the use of bioinformatics tools, in order to be directed to the list with potential to be tested against Acinetobacter baumannii. As Strategy I, the computational construction of a hybrid molecule of antibiotics from the class of β-lactams and tetracyclines was carried out, which would be an effective model against two targets in Acinetobacter baumannii, the penicillin-binding protein and the 30S ribosomal unit. This hybrid molecule was used as a model in a virtual screening based on the ligand, which obtained through a search in the PubChem database 295 molecular structures that retained characteristics of tetracyclines and β-lactams. As strategy II, through the partnership with Dr. Geisa Evaristo, went to carry out the bioprospecting of those present in the emotion felt by Phyllomedusa camba, a species of frog that has its attraction culturally despised for being tolerated in the Amazon region with therapeutic emotions. Through Strategy II, a total of 149 messages were followed. As indicated in Strategies I and II, they were used in molecular docking against the penicillin-binding protein and the 30S ribosomal unit of Acinetobacter baumannii. And the results showed promises followed by both strategy I and strategy II. Those flagged through Strategy I, due to the impossibility of acquiring them, could not be tested in vitro against A. baumannii. However, the Minimum Inhibitory Concentration (MIC) test for the injection of P. camba, obtained promising results, and pointed to a potent source of effective emission against A. baumannii.

COMMITTEE MEMBERS:
Externo à Instituição - JOÃO HERMÍNIO MARTINS DA SILVA - FIOCRUZ
Presidente - ***.429.088-** - CAROLINA BIONI GARCIA TELES - FIOCRUZ
Externa à Instituição - CLARICE MAIA CARVALHO - UFAC