Banca de DEFESA: MARCOS EDUARDO PASSOS DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARCOS EDUARDO PASSOS DA SILVA
DATE: 23/02/2024
TIME: 09:00
LOCAL: Sala Virtual Remora //bit.ly/Def_MSc_MarcosEduardo_PGBIOEXP
TITLE:

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KEY WORDS:

Pseudomonas aeruginosa. Carbapenems. Biofilm. Multiresistance

PAGES: 110
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Pseudomonas aeruginosa is among the main microorganisms causing Healthcare-Associated Infections (HAIs), standing out as an opportunistic pathogen in Intensive Care Units (ICU). The great urgency surrounding this species is due to the increasing number of strains resistant to the various antibiotics used in its antibiotic therapy, including carbepenems, which among conventional antibiotics are the main choice for the treatment of infections. In these terms, multiple resistance mechanisms are associated with P. aeruginosa, such as expression of genes encoding enzymes that inactivate or hydrolyze the antimicrobials, and virulence factors such as biofilm. Therefore, the aim of this study was to molecularly identify the mechanisms of resistance in isolates of P. aeruginosa from patients hospitalized and hospital environment of ICUs in Porto Velho/RO. The samples were collected between 2017 and 2018 from three public reference hospitals. Antimicrobial susceptibility testing was carried out by disk diffusion and molecular typing of the isolates by PFGE and MLST. A total of 216 isolates were obtained, of which 162 were from patients: Oral cavity (78); Tracheostomy (37); Axilla (24); Urine (11); Blood (6) and Wound secretion (6). Of the hospital structures there were 54: Sink (22); Faucet (19); Bed (11) and Floor (2). As for the resistance profile, the highest rate of non-susceptibility was to Imipenem with 33.7% and Meropenem with 29.6%. Of the susceptible isolates, 93% were susceptible to Polymyxin B and 89.3% to Piperaciclin-Tazobactam. The multidrug resistance (MDR) phenotype was presented by 30% of the isolates, 84.6% of which came from patients. As for the resistance genes, the highest prevalence was blaCTX-M with 38.8%, followed by blaTEM with 29.1% and blaGES with 13.9%. The presence of P. aeruginosa clones was observed in different sites of the same patient, as well as in hospital structures and patients, and in different hospitals carrying resistance genes. In addition, high-risk clones (ST111, ST235 and ST277) were found worldwide and four new STs were described in this study. Furthermore, these data serve as a warning of the risk of dissemination of this pathogen harboring resistance genes, which could have an impact on HAIs and public health problems.

COMMITTEE MEMBERS:
Externo à Instituição - ROBSON DE SOUZA LEÃO - UERJ
Externo à Instituição - IVSON CASSIANO DE OLIVEIRA SANTOS
Presidente - ***.482.422-** - NAJLA BENEVIDES MATOS - UNIR