Banca de DEFESA: GIL GUIBSON MOTA AMARAL

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GIL GUIBSON MOTA AMARAL
DATE: 17/05/2024
TIME: 08:00
LOCAL: Sala Virtual Remota (Microsoft Teams) https://bit.ly/DefMSc_GilGuibson_PGBIOEXP
TITLE:

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KEY WORDS:

SARS-CoV-2. Viral codetection. Child population.

PAGES: 78
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

The coronavirus 2019 disease (COVID-19) pandemic has affected the child population directly and indirectly. Studies that prioritize these individuals are still scarce, as well as in the Northern Region of Brazil, which concentrates the highest death rates in children from COVID-19 in the country. The aim of this study was therefore to investigate the epidemiology of SARS-CoV-2 and its co-detection with other respiratory viruses in children in the state of Rondônia. This study involved 300 patients from the Cosme e Damião Children's Hospital, aged between 0.1 months and 12 years, from November 2021 to September 2022. The analyses were carried out using the RT-qPCR technique for 17 types of respiratory virus, including SARS-CoV-2, adenovirus; bocavirus; rhinovirus; HCoV-229E; HCoV-HKU1; HCoV-NL63; HCoV-OC43; metapneumovirus; respiratory syncytial virus A and B; parainfluenza 1,2,3; influenza A (H1N1, H3N2) and Influenza B. Specimens positive for SARS-CoV-2 were sequenced by next-generation sequencing (NGS) and the strains were classified using Nextclade. Our results showed that the detection rate for SARS-CoV-2 was 7.7% (23/300), with a higher prevalence for males with 82.6% (19/23), being statistically significant (0.0253*). Children under the age of 2 had higher detection rates for viral pathogens. The association between suspected or confirmed COVID-19 relatives in the same household and patient positivity was statistically significant (<0.0001*). The most frequent symptoms were fever with 60.9% (14/23), followed by coryza with 47.8% (11/23). The symptoms coryza (0.0498*) and headache (0.0151*) were statistically significant. 21.7% (5/23) of the positive cohort were sequenced, and the Omicron variant was associated with 80.0% (4/5) of the cases analyzed, all of which were associated with the AY.99.2 strain. The quantified viral load showed a median of 6.35 log10 copies/mL, with an average of 6 days of symptoms. The detection rate for other types of respiratory virus was 44.7% (134/300), of which rhinovirus was the pathogen with the highest detection rate, with 19.4% (26/134), followed by adenovirus with 15.7% (21/134). The codetection rate with SARS-CoV-2 was 30.4% (7/23), with higher findings for rhinovirus and influenza A/H3N2, with 28.6% (2/7) respectively. Therefore, this study has provided a better understanding of the behavior of SARS-CoV-2 and other viral pathogens co-circulating in the child population of the state of Rondônia. The data obtained in this study will add to future studies needed to resolve doubts that still exist about COVID-19 in the child population.

COMMITTEE MEMBERS:
Externa à Instituição - PATRÍCIA CARVALHO DE SEQUEIRA
Externo à Instituição - LUANNA DA SILVA SOARES
Presidente - ***.482.422-** - NAJLA BENEVIDES MATOS - UNIR