Banca de DEFESA: CLAUDIA CHRISTIAN BEZERRA DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CLAUDIA CHRISTIAN BEZERRA DE SOUZA
DATE: 30/10/2024
TIME: 09:00
LOCAL: Sala Virtual Remota (Microsoft Teams) https://bit.ly/DefMSc_ClaudiaSouza_PGBIOEXP
TITLE:

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KEY WORDS:

Adulticidal; bioprospecting; larvicidal; pupicidal.

PAGES: 83
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

The mosquito Aedes aegypti is an arthropod vector of viruses such as Dengue,
Zika, Chikungunya, and urban Yellow Fever. Only Dengue and Yellow Fever
have vaccines as preventive treatments, with vector control still being a method
for suppressing arboviral diseases and preventing new outbreaks. Therefore,
discovering substances and their applicability for vector control is an ongoing
pursuit. Given this context, the objective of this study was to evaluate the
insecticidal effects of essential oil and vegetable oil on different life stages of Ae.
aegypti. Essential oil from Morinda citrifolia fruit (OE noni) and vegetable oil from
Carapa guianensis seed (OV andiroba) were used to assess ovicidal (20
eggs/treatment), larvicidal (10 L3 larvae/treatment), pupicidal (10
pupae/treatment), and adulticidal activity through the ingestion of sugar baits (20
females/treatment). The protocol adapted from the World Health Organization
was used in tests with concentrations ranging from 1000, 750, 500, 250, 125, 50,
25, 10, and 5 ppm, with distilled water and 1% ethanol controls, and three
replicates and three repetitions with different generations. The tests were
observed for 24 to 72 hours. No ovicidal effect was observed for either oil at the
tested concentrations, except for the OE noni higher concentration. The larvicidal
activity of OE noni resulted in LC50 (24 h) 90.1 ppm and LC90 (24 h) 146.1 ppm,
causing 100% mortality at concentrations from 250 to 150 ppm in the first 24
hours of the bioassay. OV andiroba had LC50 (24 h) 646.7 ppm and LC90 (24 h)
3048.3 ppm, causing 66.67% mortality at 250 ppm and 58.33% at 400 ppm after
48 hours of observation. The pupicidal activity of OE noni resulted in LC50 387.6
ppm and LC90 588.3 ppm, causing 82.22% mortality at 500 ppm and 64.44% at
450 ppm in the first 24 hours of the bioassay. OV andiroba had LC50 (24 h)
1460.02 ppm and LC90 (24 h) 2701.7 ppm, causing 98.89% mortality at 3000
ppm and 2500 ppm in the first 24 hours. No adulticidal activity was observed from
the ingestion of either oil (OE noni and OV andiroba) at the tested concentrations,
despite high ingestion. It is concluded that both oils exhibited insecticidal activity
against the larval and pupal stages of Ae. aegypti. Additionally, the consumption
of sugar baits containing both oils did not result in adult mortality at the tested
concentrations, regardless of the high level of engorgement.

COMMITTEE MEMBERS:
Externo à Instituição - JOELMA SOARES DA SILVA - UFMA
Presidente - 1530806 - ALEXANDRE DE ALMEIDA E SILVA
Externa à Instituição - ROSEMARY APARECIDA ROQUE - INPA