Banca de DEFESA: HILLQUIAS MONTEIRO MOREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : HILLQUIAS MONTEIRO MOREIRA
DATE: 12/12/2024
TIME: 08:30
LOCAL: Sala Virtual Remota (Microsoft Teams) https://bit.ly/DefMSc_HillquiasMoreira_PGBIOEXP
TITLE:

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KEY WORDS:

Oropouche; OROV; epidemiology; phylogeny

PAGES: 80
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

The Oropouche virus - OROV, is characterized as a re-emerging arbovirus of great concern to public health due to its capacity for genetic rearrangement, and since its discovery has been responsible for several outbreaks of acute fever identified in Central and South American countries, with at least 500,000 reported cases. The western Amazon region is already notorious for outbreaks of acute febrile illnesses, for which the lack of specific diagnoses for different pathogens makes patient management difficult. The OROV was already recorded in the region in the 1990s, and this is the first study since that record to detect individuals with acute febrile illness using a screening test to exclude Zika, dengue and chikungunya, confirming by sequencing the circulation of the Oropouche virus in the state of Rondonia and the border. Reports of the incidence of this virus are intrinsically favored by environmental and climatic conditions, which can favor the increase and distribution of the vector population to areas of human traffic. In addition, there is a problem related to the lack of distribution of the specific diagnosis established in routine screening for arboviruses in Brazil. Thus, through molecular techniques of RT-qPCR (Reverse Transcription quantitative Polymerase Chain Reaction) used as a primary exclusion methodology for Zika, dengue and Chikungunya, and evaluating profiles of OROV co-infections with other arboviruses. Sanger sequencing confirmed the circulation of the Oropouche virus in the state of Rondonia and the border. 20.99% (421/2006) positive OROV cases were identified, with detections of 1.43% (6/421) for co-infections with dengue (serotypes 1 and 2) circulating in border locations in western Amazonia, among serum samples, with 95.81% (366/382) of the cohort characterized as infected individuals with reported symptoms, mainly fever, headache, myalgia, and back pain. Among the positive samples, it was possible to obtain a total of 3.40% (13/382) of samples for analysis of the S and M segments of Oropouche, which showed similarities with Brazilian sequences. It was thus possible to verify the circulation of OROV in Rondonia and border areas, strengthening genomic surveillance of emerging and re-emerging viruses.

COMMITTEE MEMBERS:
Externo à Instituição - MONICA BARCELLOS ARRUDA - FIOCRUZ
Presidente - 1786399 - DHELIO BATISTA PEREIRA
Externa à Instituição - ZORAIDA DEL CARMEN FERNADEZ - FIOCRUZ