Banca de QUALIFICAÇÃO: RENATA BISPO SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : RENATA BISPO SANTOS
DATE: 11/10/2022
TIME: 08:00
LOCAL: Sala Virtual Remota
TITLE:

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KEY WORDS:

Leishmaniasis, Leishmania RNA virus 1, diagnosis.

PAGES: 73
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Cutaneous leishmaniasis (TL) is a disease of wide geographic distribution, occurring in
four continents: Americas, Asia, Europe and Africa. In Brazil, eight species of
Leishmania causing TL have already been identified, all occurring in the North region.
L. braziliensis and L. guyanensis are the most frequent species in cases of TL and some
strains have a viral endosymbiont, LRV1, which has been associated with the clinical
evolution of TL from the cutaneous to the mucosal form. Laboratory diagnosis is made
through direct parasitological examination, immunological and molecular techniques
such as PCR and its variations. In view of the molecular techniques that have been used
in the diagnosis of TL, qPCR optimizes the diagnosis, as it associates greater sensitivity,
specificity, with efficient and quantitative detection. Therefore, this study aimed to
evaluate the use of qPCR approaches to quantify the parasite and viral load in the
routine diagnosis of TL in patients treated at the Centro de Medicina Tropical de
Rondônia (CEMETRON), using lesion scraping samples, blood and healthy mucosa.
Only patients with skin lesions suspected of having cutaneous leishmaniasis were
included in this study. All samples collected were submitted to genetic material
extraction, reverse transcription (to LRV1 RNA) followed by qPCR for detection and
quantification of Leishmania with the 18S rDNA target and LRV1 with the ORF1 target.
Of 100 samples analyzed for Leishmania detection and quantification, 56 skin lesion
samples were positive using the 18s rDNA target, two blood samples and 11 healthy
mucosa samples were positive. From the results obtained with the qPCR for the 18S
rDNA target, assays for detection and quantification of LRV1 were performed. Fifty-six
samples were processed, 26 of which were skin lesion samples, four blood samples and
11 healthy mucosa samples were positive for LRV1. The results obtained with the
methodology used showed a substantial confidence index, which demonstrates that the
method is robust and reliable to be used in the routine diagnosis of TL.

COMMITTEE MEMBERS:
Presidente - ***.429.088-** - CAROLINA BIONI GARCIA TELES - FIOCRUZ
Externo à Instituição - FELIEP DUTRA RÊGO
Externo à Instituição - OTACILIO DA CRUZ MOREIRA