Banca de DEFESA: CLEICIAINARA BAGIO LOVO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CLEICIAINARA BAGIO LOVO
DATE: 14/03/2023
TIME: 14:00
LOCAL: Sala Virtual
TITLE:

Molecular epidemiology of Candida species isolated from patients in the region of Porto Velho, RO, Brazil.

KEY WORDS:

Candida albicans. Phospholipase. Fluconazole. Itraconazole. Porto Velho-RO.

PAGES: 98
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Candida spp. they colonize the human organism in a commensal way, only, depending
on the states of the host, these microorganisms can to comport as opportunistic agents
and cause from superficial infections to systemic infections. These infections result in
prolonged hospitalizations, increased hospital costs, and high rates of morbidity and
mortality. Despite this, when it comes to the northern region of Brazil, studies on
candidiasis are scarce, and in Rondônia, they are non-existent. Therefore, the present
study aimed to carry out the molecular characterization of clinical isolates of Candida
spp. obtained from patients in the Porto Velho/RO region, as well as to phenotypically
determine virulence factors and antifungal susceptibility. For this, the isolates were
obtained from clinical specimens of patients attended at the Laboratory of Medical
Mycology of the Centro de Pesquisa em Medicina Tropical de Rondônia (CEPEM/RO),
which were first identified through the CHROMagar Candida medium. Molecular
characterization of the species was performed through PCR of the ITS region of fungal
rDNA coupled to genetic sequencing. The disk diffusion method was used following
the recommendations of the M44-P protocol to determine antifungal susceptibility to
nystatin, amphotericin B, fluconazole, itraconazole and miconazole. To determine the
hemolysin activity, the sheep blood agar assay was used and, to determine the
phospholipase activity, the egg yolk agar method was used. The present study
evidenced the presence of five species of Candida as causing infections in patients in
the Porto Velho/RO region, of which C. albicans 22/31 (71%) was the most frequent,
followed by C. tropicalis with 6/31 (19,4%), C. parapsilosis with 1/31 (3,2%),
Cyberlindnera jadinii with 1/31 (3,2%) and C. duobushaemulonii with 1/31 (3,2%).
Resistance to fluconazole was observed in 26/30 (86,7%) isolates and to itraconazole
in 24/30 (80%) isolates, mainly in species of C. albicans, C. tropicalis and
Cyberlindnera jadinii, evidencing a reduction in the sensitivity of Candida spp. to first
option triazole antifungals. Besides, the present study demonstrated that 30/30 (100%)
of Candida isolates were hemolysin producers and that only C. albicans 9/22 (40,9%)
and Cyberlindnera jadinii 1/1 (100%) were phospholipase producers. These results
inedited provide important information for the management of infections in relation to
strategies of the treatment, control and prevention of the cases of candidiasis
diagnosed in the region.

COMMITTEE MEMBERS:
Externa à Instituição - ORMEZINDA CELESTE CRISTO FERNANDES
Externa à Instituição - ANDRELISSE ARRUDA - FIOCRUZ
Presidente - 2148421 - CHRISTIAN COLLINS KUEHN