Banca de DEFESA: AMÉLIA MACARLE SILVA DE SOUSA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : AMÉLIA MACARLE SILVA DE SOUSA
DATE: 05/11/2024
TIME: 09:00
LOCAL: Sala Virtual Remota (Microsoft Teams) https://bit.ly/DefMSc_AmeliaSouza_PGBIOEXP
TITLE:

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KEY WORDS:

Ecology; Immatures; Adults; Reproduction; Preference.

PAGES: 44
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

The genus Mansonia Blanchard, 1901 comprises 25 species classified within the
tribe Mansoniini, divided into two subgenera: Mansonia (Mansonia) (with 15
species) and Mansonia (Mansonioides) (with 10 species). Only Mansonia
(Mansonia) occurs in the Neotropical region. During the oviposition process, the
eggs are deposited on the underside of the leaves of the plants used as
substrates. Some studies have highlighted the association of Mansonia species
with aquatic macrophytes Eichhornia crassipes and Pistia stratiotes in Asia and
North America. However, laboratory studies on the oviposition preference of
Mansonia in Brazil have not been found. Therefore, this study aimed to analyze
the oviposition preference of Mansonia humeralis in aquatic macrophytes under
two experimental conditions in the laboratory. For this, female Mansonia
humeralis were collected by human-protected bait and placed in plastic cages,
being fed human blood for 15 minutes in the field. Three days after the blood
meal, the females were identified and placed inside BugDorm cages/traps (L 32.5
x W 32.5 x H 32.5 cm) with rectangular plastic basins containing 1.8 liters of
filtered water for five days, containing the specific substrates of the treatments,
divided into two distinct groups. The Isolated Substrates group (IS): (i) E.
crassipes plant; (ii) P. stratiotes plant; (iii) Salvinia spp., and (iv) abiotic substrate
expanded polystyrene (EPS). The Combined Substrates group (CS) consisted of
(v) all plants + EPS (MIX). After this period, the oviposition substrates were
inspected for posture quantification and egg and larval counting. A total of 886
females were used, and 66 oviposition’s (7.46%) were recorded. Among the 66
recorded oviposition’s, 40 (60.6%) were in the IS group, and 26 (39.4%) in the
CS group. Regardless of the experimental group, no females oviposited on E.
crassipes. In the IS group, of the 40 recorded oviposition’s, 50% of the females
oviposited on Salvinia sp., 35% on P. stratiotes, and only 15% on EPS,
suggesting a preference for these plants compared to the inert substrate (EPS).
In the SC group, of the 26 recorded oviposition’s, 42% were on Salvinia sp., 34.6%
on P. stratiotes, and 23% on EPS, with no differences in the frequency of
oviposition among these substrates. The results suggest that Mansonia
humeralis does not oviposit on E. crassipes but oviposits more frequently on the
other plants compared to EPS under the experimental laboratory conditions used.
A total of 6,054 eggs were obtained without induction, showing no significant
difference between the plants Salvinia (92) and Pistia (88) and the abiotic
substrate (94). A total of 2,568 larvae were obtained, with a hatching rate of 42%.
There was a significant difference between the number of larvae hatched on EPS
(60) compared to the plants, but no difference between Salvinia (45) and Pistia
(27). Regarding larval preference, a significant difference was observed in larval
attachment to Pistia (88) compared to the other substrates, Eichhornia (45) and
Salvinia (23).

COMMITTEE MEMBERS:
Presidente - 1530806 - ALEXANDRE DE ALMEIDA E SILVA
Externo à Instituição - FRANCISCO AUGUSTO DA SILVA FERREIRA - INPA
Externo à Instituição - LUIZ GUILHERME SOARES DA ROCHA BAUZER