Banca de DEFESA: LIZIANE ROLIM DANTAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LIZIANE ROLIM DANTAS
DATA : 04/11/2019
HORA: 09:00
LOCAL: Programa de Pós Graduação em Biologia
TÍTULO:

EVALUATION OF PHENOTYPIC AND GENETIC DIVERSITY OF ISOLATES OF THE COMPLEX Mycobacterium abscessus FROM PULMONARY INFECTIONS IN THE STATE OF RONDONIA

PALAVRAS-CHAVES:

Keywords: Mycobacteria not causing tuberculosis; M. abscessus; rpoB hsp65 ITS1 16S pulmonary infection Rondonia

PÁGINAS: 90
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
RESUMO:

Non-tuberculosis mycobacteria (NTM) have been associated with
with a variety of diseases, including lung, lymph node infections,
and nosocomial infections, including outbreaks. The Mycobacterium Complex
abscessus is composed of three subspecies: M. abscessus subsp. abscessus,
M. abscessus subsp. bolletii and M. abscessus subsp. massiliense, which are
pathogenic, fast-growing mycobacteria (MCR)
distribution in the environment. Precise diagnosis of the subspecies of the
M. abscessus complex is of great importance due to
clinics and specific therapeutic conduct for each infectious agent. At the
from 2008 to 2017 were identified by LACEN / RO 170 samples
positive for NTM in the state of Rondônia, of these 62 (35.8%) were
belonging to the M. abscessus complex.
most frequently identified. In order to evaluate characteristics
phenotypic and genotypic isolates from isolates previously identified as
belonging to the M. abscessus complex and to separate them at subspecies level,
phenotypic tests were performed such as: growth time, morphology of the
colonies, pigment presence, chord factor formation, molecular tests
using the RFLP / PRA / hsp65 methodology, sequencing regions of the
16S rRNA, rpoB and hsp65 genes and PCR from erm (41) gene from 16 isolates
from sputum samples from patients with infection
between 2008 and 2017 in the state of Rondônia. Of 16
bacterial isolates analyzed in PRA hsp65, it was possible to confirm that 15
belonged to the M. abscessus complex, but one isolate showed a pattern of
distinct band from the other mycobacterial isolates. The analysis of
sequencing of the 16S rRNA gene, separated the isolates only at
Partial sequencing of the rpoB and hsp65 genes identified
M. abscessus Complex at the species level, where 18.7% were
identified in M. abscessus subsp. abscessus, 37.5% were identified
as M. abscessus subsp. bolletti, 37.5% were identified as M.
abscessus subsp massiliense and 6.25% identified as M. avium subsp.
hominissuis. PCR amplification of the erm gene (41) helped in the identification
subspecies and confirmed the identification of five M. abscessus isolates
subsp. massiliense, but there was a case of M. abscessus subsp. massiliense
identified by sequencing and not by the erm gene (41). The results of the
analyzes have shown that the phenotypic evidence currently used is not
differentiate mycobacteria from the M. abscessus complex and that the
partial sequencing of the rpoB and hsp65 genes were able to distinguish the
three subspecies, but as there are reports of horizontal gene transfer,
Using these two markers, the need to identify
new targets or combination of targets to overcome the transfer problem
of genes between M. abscessus complex mycobacteria.

MEMBROS DA BANCA:
Presidente - 087.927.277-51 - GABRIEL EDUARDO MELIM FERREIRA - UNIR
Externo ao Programa - 1287751 - MARIA MANUELA DA FONSECA MOURA
Externo à Instituição - ARTEMIR COELHO DE BRITO - MS