Banca de DEFESA: MICHELLI SANTOS DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MICHELLI SANTOS DA SILVA
DATE: 27/05/2022
TIME: 14:00
LOCAL: Sala Virtual Remota
TITLE:

USE light-emitting diodes (LEDs) in the capture of  sand flies (Diptera, Psychodidae), molecular detection of leishmania and blood meal sources used by females in a locality in the municipality of Porto Velho, Rondônia, Brazil

KEY WORDS:

Light source. Vectors. Leishmaniasis. Infection. Traps.

PAGES: 72
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Sand flies are dipteran insects that are important for acting as vectors of pathogens, including Leishmania species. One of the main strategies to understand these insects is based on monitoring from entomological surveys. Light traps are among the main methods for capturing sand fly species as they generally respond to light stimuli. In recent years, studies have effectively adapted light-emitting diodes (LEDs) to traps. However, no study has evaluated the effectiveness of LEDs in capturing of sand flies in the Amazon biome. Based on this assumption, the present study aims to evaluate the influence of LEDs on the capture and diversity of sand flies in Porto Velho, Rondônia, Brazil, in addition to identifying potential Leishmania vectors and the blood meal sources used by females. Thus, sand flies were collected with HP light traps using blue, green, red and ultraviolet LEDs, as well as incandescent light as a control using the Latin square method. A total of 1,993 individuals were collected, distributed in 52 species belonging to 12 genera. The most abundant species in this study were Trichophoromyia ubiquitalis (n=365), Bichromomyia flaviscutellata (222), Psychodopygus davisi (148), Nyssomyia Antunesi complex (128). The incandescent light HP captured the largest number of individuals (n=590), followed by blue (471), green (452), ultraviolet (281) and red (150) LED traps. A PERMANOVA test was applied and there was no difference in the composition of the sandfly fauna by the different light sources (Pseudo F = 1.29, p = 0.14). This same pattern was also observed when applying the NMDS, in which no difference was observed in the composition of the sandfly species (Stress 0.18). The number of males 1,057 (54.4%) was higher in relation to females 887 (45.6%) in most light sources, but a greater number of females in relation to males was observed in the trap with the blue LED. For the natural infection, 32 pools of 275 females of 10 species were analyzed. None of the pools was positive for Leishmania detection, however amplification was observed for Endotrypanum spp. referring to the pool of Ny. fraihai. The following feeding sources were observed: Tamandua tetradactyla and Choloepus hoffmanni in females from the Antunesi complex (12) and in Ny. richardwardi (1), Proechimys gardneri in Bi. flaviscutellata (2) and Homo sapiens in Bi. flaviscutellata (1). The data obtained indicate that blue and green LEDs can be as efficient as incandescent light, that the sandflies in this study area use several blood meal sources and that other trypanosomatid species can be found in the sand flies. The data obtained can help in future studies in the region.

BANKING MEMBERS:
Externo à Instituição - ANDREY JOSÉ DE ANDRADE - UFPR
Externo à Instituição - FELIEP DUTRA RÊGO
Presidente - 838.495.134-91 - JANSEN FERNANDES MEDEIROS - UNIR