Banca de QUALIFICAÇÃO: SAYONARA DOS REIS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : SAYONARA DOS REIS
DATE: 09/06/2022
TIME: 08:00
LOCAL: Sala Virtual Remota
TITLE:

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KEY WORDS:

Leishmania lindenbergi; Leishmania naiffi; Genetic diversity.

PAGES: 74
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Leishmaniasis is an infectious, non-contagious disease caused by the parasite of de genus Leishmania. The North Region has the highest number cases of Tegumentary Leishmaniasis (TL) in Brazil, and in the state of Rondônia, the seven dermotropic species of this parasite recorded in the country were identified. However, the species identification is still a challenge for laboratory routines. In this context, the present work seeks to evaluate the identification of Leishmania spp. in samples with an inconclusive enzymatic restriction profile or identified as L. guyanensis, using RFLP approaches and nucleotide sequence analysis of a region of the hsp70 gene. Two groups of samples from patients diagnosed with TL by the Genetic Epidemiology Laboratory between the period 2012 to 2019 were selected, divided into samples identified by RFLP of L. guyanensis (group I) and samples with an inclusive restriction profile (group II). The samples were submitted to conventional PCR for 234bp hsp70 target, and then sent to the sequencing platform. The hsp70-PCR-RFLP assays were performed using a panel of three restriction enzymes (HaeIII, BstUI and MboI). Among the 110 samples selected, only 66 tested positive for PCR. Of these, 30 samples from group I and 36 samples from group II. Analyzing the sequences obtained by sequencing, it was possible to identify all species from all samples using the BLAST algorithm. Analyzing the results obtained from the two techniques, in group I, three species were identified, L. braziliensis, L. guyanensis and L. lindenbergi, in group II, five species were identified, L. braziliensis, L. guyanensis, L. amazonensis, L. naiffi and L. infantum. In 13 sample from 13 patients did not obtain results by RFLP method were identified as Leishmania spp. While more studies are still needed connecting Leishmania species to response to treatment and clinical manifestations of this disease, the identification of species is recommended to define the best follow-up and therapeutic management. In great biodiversity regions as the Amazon, the diversity of species present makes this step essential for a better understanding of epidemiology and prognosis of disease. The results obtained performed demonstrate that RFLP method and the analyzing the nucleotides of the molecular target of this study, it is possible to identify Leishmania species recorded in Brazil. Despite the limitations presented, either in terms of resolution or laboratory structure, both approaches can be used in a complementary way to identify the Leishmania species evaluated

BANKING MEMBERS:
Externo à Instituição - EDUARDO DE CASTRO FERREIRA
Externa à Instituição - CAROLINE RICCE ESPADA
Presidente - 218.429.088-20 - CAROLINA BIONI GARCIA TELES - FIOCRUZ