Banca de DEFESA: MARIA JOSE DE SOUZA SARAIVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARIA JOSE DE SOUZA SARAIVA
DATA : 17/09/2019
HORA: 09:00
LOCAL: programa de Biologia Experimental
TÍTULO:

EVALUATION OF THE INFLUENCE OF PARASITEMIA ON MARKERS OF PLACETARY ACTIVATION ANALYZED BY FLOW CYTOMETRY IN PLASMODIUM VIVAX INFECTED PATIENTS

PALAVRAS-CHAVES:

Key words: Plamodium vivax, platelet activation, flow cytometry.

PÁGINAS: 90
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
RESUMO:

Malaria is still one of the major causes of morbidity and mortality in many tropical and subtropical countries. Understanding the pathogenesis associated with Plasmodium vivax malaria has been a challenge in recent years, which makes it study the pathogenic mechanisms of this species. Given the character displayed by vivax malaria, it becomes relevant to study the influence of platelet activation in disease severity. In this sense, the present work investigated the levels of important platelet surface proteins in patients with acute Plasmodium vivax malaria with varying degrees of parasitemia. O The aim of this study was to standardize the techniques to evaluate the expression of platelet activation markers and apply them in the study of malaria, as well as standardize the antibody titration technique, where it was possible to obtain the concentration antibodies that were used. Eleven individuals were evaluated, of which 10 were Plasmodium vivax infected individuals and one were non-Plasmodium infected. In this stage the biomarkers αIIbβ3 portion αIIb - CD41a), P-Selectin (CD62P). It was also analyzed the percentage of the isolation of platelets of all individuals. In both groups (infected individuals and not CD41a expression was observed, representing an average of 98.5% What is expected once, is that this is the first glycoprotein present on the surface. Regarding the expression of P-Selectin (CD62P), in sick individuals there was a low expression of P-Selectin, ranging from 0.28 to 1.07. How much platelet isolation was observed within the platelet gate, an average 97% purity of this isolation. A total of 50,000 events have been acquired. per tube using the Celesta® flow cytometer (Becton Dickinson). For the acquisition and data analysis, the Flowjo software was used, as recommended by the manufacturer and finally the appropriate statistical analysis will be applied to future experiments.

MEMBROS DA BANCA:
Interno - 643.265.191-34 - DEUSILENE SOUZA VIEIRA - UNIR
Presidente - 1786399 - DHELIO BATISTA PEREIRA
Externo à Instituição - LUAN FELIPO BOTELHO SOUZA - FIMCA