Banca de DEFESA: JEANE DO NASCIMENTO MORAES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JEANE DO NASCIMENTO MORAES
DATA : 26/11/2021
HORA: 15:00
LOCAL: Sala Virtual Remota
TÍTULO:

Structural and functional characterization of a Cathepsin D Enzyme Complex and a Bothrops jararacussu phospholipase A2.

PALAVRAS-CHAVES:

Complex, cathepsin D, phospholipase A2, enzyme complex.

PÁGINAS: 59
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Enzimologia
RESUMO:

Cathepsin D (CatD) is a lysosomal proteolytic enzyme expressed in almost all tissues and organs. It is a multifunctional enzyme responsible for important proteolytic events for the regulation of biological processes such as cell cycle migration and differentiation, tissue remodeling, neuronal growth, ovulation and apoptosis. Its overexpression and hypersecretion have been correlated with cancer aggressiveness and tumor progression, stimulating cancer cell proliferation, fibroblast growth and angiogenesis. Furthermore, some studies report its participation in neurodegenerative diseases and inflammatory processes. The search for new inhibitors from natural products can be an alternative against the harmful effects of the action of this enzyme. For this, we investigated the interaction of CatD with snake venom toxins in an attempt to find inhibitory molecules. However, it was observed that CatD interacts with phospholipases A2 (PLA2) from snake venoms, forming a stable complex without its activity being affected by specific toxins or inhibitors (pepstatin A). The formation of the toxin-protease complex was corroborated by molecular docking assays and evidenced by two-dimensional electrophoresis. The functional characterization of the CatD/BthTx-II complex was evaluated through proteolytic activity on the casein substrate, where the formation of an enzymatically active complex was verified. Therefore, this work describes for the first time an enzymatic complex between a lysosomal protease (CatD) and a snake venom to in (PLA2), whose effects were previously unknown.

MEMBROS DA BANCA:
Interno - 218.429.088-20 - CAROLINA BIONI GARCIA TELES - FIOCRUZ
Externo à Instituição - DANIEL DE CARVALHO PIMENTA - IB/SP
Interno - 457.646.382-91 - FERNANDO BERTON ZANCHI - UNIR
Externo à Instituição - LEANDRO SOARES MOREIRA - UNIR
Presidente - 1522184 - LEONARDO DE AZEVEDO CALDERON