Banca de DEFESA: AUREA VIEIRA TEIXEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : AUREA VIEIRA TEIXEIRA
DATE: 20/05/2022
TIME: 14:30
LOCAL: Sala Virtual Remota
TITLE:

BAIT STATIONS AS A TOOL FOR THE CONTROL OF Aedes aegypti (DIPTERA: CULICIDAE): EVALUATION OF MODELS AND COMPONENTS OF SUGAR TOXIC ATTRACTIVE BAITS (ATSB) IN THE LABORATORY

KEY WORDS:

Phagostimulants. Combined Insecticides. Ivermectin. Dinotefuran.

PAGES: 88
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Aedes aegypti is an important vector of arboviruses such as dengue, yellow fever, chikungunya and Zika, and the absence of vaccines or treatments for most of them makes vector control the main alternative to reduce the transmission. Among vector control methods, chemical control is widely used, and includes new approaches, such as Attractive Toxic Sugar Baits (ATSBs). The ATSBs aim to supply an insecticide in an attractive sugar solution, using the natural sugar feeding habit by both males and females to induce mosquitoes to ingest the bait. In addition to the versatility of this bait in relation to its composition, previous studies have indicated the potential of offering ATSB using bait stations (BSs), making it an additional lowcost tool. Therefore, the objectives of this work were to evaluate different models of BSs in the laboratory and to estimate the Lethal Concentrations (LCs) of dinotefuran (DNT) alone and combined with ivermectin (IVM), in males and females of Ae. aegypti. The BS models were: Artificial plant, pet bottle, ovitrap and resting box, sprayed with two solutions: 10% sucrose (SB) and 10% sucrose and guava juice (ASB). The experiments were carried out in screened cages with 100 fasted mosquitoes (24h males and 48h females), during 6 hours. Later the engorged mosquitoes were separated according to the degree of engorgement. To calculate the DNT LCs, ATSBs were prepared with 70% sucrose and increasing concentrations of DNT. For the combination of insecticides experiments, increasing concentrations of DNT were prepared combined with 0.312ppm IVM and increasing concentrations of IVM combined with 1ppm of DNT. Subsequently, synergism ratios were calculated. The engorgement of the ASB (7.0 - 40%) was much lower than SB baits (50 - 80%). All BSs can be used under the conditions tested, as there were no differences in engorgement and degrees of engorgement of mosquitoes between them. ATSBs with DNT did not affect general engorgement or degree of engorgement that did not varied with increasing concentrations of this insecticide. The mortality of females, in general, was lower than that of males, especially at low concentrations and the CL90 of males and females was 35.6 and 56.0ppm, respectively. The combination of insecticides in ATSBs did not affect the engorgement (80%) and a synergistic effect was observed in mosquito mortality, especially for the combination of IVM + DNT in low concentration. SB and ATSB baits applied to all tested BSs were engorged by males and females of Ae. aegypti. In addition, the combination of insecticides IVM+DNT significantly reduced the CLs of insecticides required for use in ATSBs

BANKING MEMBERS:
Externa à Instituição - ROSEMARY APARECIDA ROQUE - INPA
Presidente - 1530806 - ALEXANDRE DE ALMEIDA E SILVA
Externo à Instituição - ELIS PAULA DE ALMEIDA BATISTA - UFMG