Banca de DEFESA: ALESSANDRA DA SILVA BASTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ALESSANDRA DA SILVA BASTOS
DATE: 05/10/2022
TIME: 14:00
LOCAL: Sala Virtual Remota
TITLE:

.

KEY WORDS:

Locomotor activity, Anopheles darlingi, circadian rhythms, blood feeding,
insemination.

PAGES: 73
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

In mosquitoes, behavioral patterns with circadian periodicity could be observed
through locomotor activity, because it corresponds to a behavior such as foraging
mating and oviposition. Habits that are fundamental to its life cycle and that are
related to the pathogens transmission to humans. In order to assess the locomotor
activity of the main malaria vector in the Amazon region, Anopheles darlingi, this
study aimed to (i) compare the locomotor activity between inseminated and not
inseminated females; (ii) to evaluate the effect of the blood feeding on the locomotor
activity of inseminated and not inseminated females; (iii) to evaluate the locomotor
activity of mosquitoes infected and not infected by Plasmodium vivax. For the
experiments, An. darlingi females from the Platform of Production and Infection of
Malaria Vectors – PIVEM/FIOCRUZ – RO, with six days of age and treated with 15%
honey solution were used. The experimental groups were separated in not
inseminated females (NINS), generated from cages which male mosquitoes were
removed immediately after emergence, and from inseminated females (INS) from
cages which there was copulation. Subsequently, the other groups were formed by
NINS and INS females without and with blood feeding (NINS SRS x NINS CRS; INS
SRS x INS CRS). And finally, females fed with blood sample from a malaria donor (P.
vivax+
) and females fed an aliquot of this sample submitted to inactivation of the
parasites (42oC for 30 min) (P. vivax-

). Blood feeding was performed through the
artificial membrane feeding Hemotek® after previous fasting. The monitoring of
locomotor activity were performed in the Flybox equipment for up to six days, with the
internal photoperiod in LD with 12 h of light and 12 h of dark. The locomotion activity
was determined from the images of FlyBox through the software WebCan Image
Save – 1.11, converted into video and quantified through PySoLo (v1.1) and
analyzed the periodicity, rhythmicity in the software MatLab® version R2015b. The
graphs generated in Excel and the statistical analysis performed in the GraphPad
Prism software version 9.1. 0. The t Student and Mann-Whitney test were applied to
compare the average locomotor activity in the time intervals: 24h, photophase,
scotophase, ZT12 and ZT0. Circadian periodicity was close to 24 h and rhythmicity
was above 50%. The locomotor activity was bimodal pattern, with a main peak called
nocturnal peak (ZT12) and a second less pronounced peak called diurnal peak
(ZT0). When comparing the insemination states, a reduction in the activity of the INS
group was recorded in ZT12 the first two days and the photophase of the third day in
relation to NINS group. The activity of the NINS CRS group was reduced in the 24-
hour cycle, scotophase and ZT12 the first two days of analysis, to first the
photophase and in the ZT0 of the three days of analysis, returning to an activity
similar to NINS SRS group on the third day. For the INS CRS group there was a
reduction in activity in the 24-hour cycle, phtophase and scotophase of the first and
third day, and in ZT0 and ZT12 of the three days of analyzeis in relation to the INS
SRS. In the group P. vivax+ the activity was reduced in the photophase of the first
and second day. We conclude that, like other vectors of the genus, An. darlingi has
nocturnal habits and that physiological conditions influence the locomotor activity of
this vector.

COMMITTEE MEMBERS:
Externo à Instituição - LUIZ GUILHERME SOARES DA ROCHA BAUZER
Presidente - 1530806 - ALEXANDRE DE ALMEIDA E SILVA
Externo à Instituição - MAISA DA SILVA ARAÚJO - FIOCRUZ