Banca de DEFESA: TALIA IASMIN PEREIRA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : TALIA IASMIN PEREIRA DA SILVA
DATE: 05/10/2023
TIME: 08:30
LOCAL: Sala Virtual Remora https://bit.ly/DefMSc_TaliaSilva_PGBIOEXP
TITLE:

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KEY WORDS:

Plasmodium vivax. P53 protein. IFN-γ. Real-time PCR. Western Amazon

PAGES: 77
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Studies carried out in vivo and in vitro demonstrated that the P53 protein and the cytokine IFN-γ may be involved in protective mechanisms against Plasmodium infection. The objective of this study was to analyze the expression level of the TP53 and IFNG genes in infection caused by P. vivax in different parasitemias in patients treated at the Tropical Medicine Research Center – CEPEM. A total of 52 samples were collected, which were classified into 4 groups with different parasitemias: +/- (half a cross), + (one cross), ++ (two crosses) and +++ (three crosses). Extraction and purification of total RNA from whole blood and cDNA synthesis were performed. The optimal primer concentration and cDNA efficiency were determined to standardize the real-time PCR technique. To evaluate the variation in expression, the comparative Ct methodology was used, given by the search ΔCT: Ct gene of interest – Ct of the control gene. Ct values of the TP53 and IFNG genes were obtained, using the GAPDH gene as an endogenous control.

Note a reduction in TP53 gene expression in patients with high parasitemia +++ (three crosses) compared to those with lower parasitemia. However, no statistically significant difference was found when comparing the other groups to each other. Expression of the IFNG gene did not vary significantly with different levels of parasitemia. The results support studies that indicate that the parasite can suppress P53 levels in the host to continue its life cycle.

COMMITTEE MEMBERS:
Externa à Instituição - JULIANE CORRÊA GLÓRIA
Externa à Instituição - ANNA CAROLINE CAMPOS AGUIAR - UNIFESP
Presidente - ***.646.382-** - FERNANDO BERTON ZANCHI - UNIR