Banca de DEFESA: ANA MAISA PASSOS DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA MAISA PASSOS DA SILVA
DATE: 25/10/2023
TIME: 14:00
LOCAL: Sala Virtual Remora
TITLE:

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KEY WORDS:

SARS-CoV-2; host; genetic role.

PAGES: 105
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

SARS-CoV-2 has been characterized as a virus that has generated great public health commotion due to its scenario. For the different clinical manifestations, components of the immune system constitute a huge population of proteins, associating themselves to a large line of defense related to innate and adaptive responses in viral infections. As originators, the performance and the genetic role in the host immune response delimit specific points of investigation that will demonstrate the association of a variety of potential genes of immunogenic behavior, as well as biomarkers that would be linked to the severity of clinical pictures. Thus, this research aimed to identify potential biomarkers in the worsening of the disease caused by SARS-CoV-2 in patients in Western Amazon. For this purpose, biological materials were collected from 223 individuals diagnosed positive for SARS-CoV-2 obtained by molecular biology and/or antigen testing, and from 20 individuals as a control population with negative diagnosis for SARS-CoV-2. Real-time PCR techniques were used for human genotyping of the targets rs2070788, rs4702, rs76635825, rs540856718, rs35803318, rs12979860 and rs16899066, as well as gene expression of the markers ACE2, HLA-A, HLA-B, IFN 3/2, IL-6 and TMPRSS2. For the analysis of genetic results, the allele and genotype frequencies were assigned along with the Hardy Weinberg Equilibrium. The other results were tabulated and calculated according to Fisher's exact test. In the study it was possible to verify that rs12979860 is already a highly adapted and disposed polymorphism among the population, as well as rs540856718 which had 100% identification of the CC genotype among all those analyzed. At the same time, the other SNPs were found to have genetic imbalance for this population, especially rs2070788 with the G allele substitution present in a large portion of mapped people. The results regarding gene expression showed quite high levels among the moderate and severe groups. The ACE2 gene showed no correlation with the disease. In the control population, the levels were commonly stable. TMPRSS2 and IL-6 performed better in gene expression analysis than the others. Thus, it was seen that there is possibly an association regarding the role of rs2070788 in SARS-CoV-2 infection, just that the polymorphism had p<0.05, being significant in the comparison of the clinical outcomes analyzed. As well as the correlation of overexpression of the TMPRSS2 gene among the more aggravated groups, in addition to the relationship of the immune response of IL-6 and the IL-6 gene verified.

COMMITTEE MEMBERS:
Externa à Instituição - RAQUEL DA HORA BARBOSA - UFF
Presidente - ***.265.191-** - DEUSILENE SOUZA VIEIRA - UNIR
Externa à Instituição - MICHELLE DE OLIVEIRA E SILVA - FIOCRUZ