Banca de DEFESA: JORGE LUIS VIDAL CAMA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JORGE LUIS VIDAL CAMA
DATE: 29/08/2025
TIME: 09:00
LOCAL: Campus de Rolim de Moura- sala 21
TITLE:

MULTICLASS ANALYSIS OF PESTICIDE RESIDUES IN TAMBAQUI (COLOSSOMA MACROPOMUM), WESTERN AMAZON, USING THE MODIFIED QUECHERS METHOD COMBINED WITH LC-MS/MS AND ELISA

KEY WORDS:

Public Health; Food Safety; Ecotoxicology; Pesticides; Environmental Contamination

PAGES: 48
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

This study aimed to investigate the presence of pesticide residues in tambaqui (Colossoma macropomum) cultivated in fish farms in Rondonia, Western Amazon, and to assess their environmental and public health implications. Advanced analytical methods, such as LC-MS/MS, ELISA, and modified QuEChERS, were used for the detection of glyphosate, its metabolite AMPA, and other pesticides in water and muscle tissue samples, in addition to histopathological analyses of fish gills. The results showed that AMPA was present in 19% of surface water samples, with concentrations ranging from 1.013 to 2.312 μg/L, mainly during the dry season, while glyphosate was detected only at levels below the quantification limit. Histopathological analysis revealed severe lesions in the gills of exposed fish, including hyperplasia, edema, degeneration, and tissue necrosis, with a mean Histopathological Index (HI) of 10.7 in exposed fish compared to 7.8 in non-exposed ones. In fish muscle, pesticide residues were detected in 48.5% of the fish farms analyzed, with chlorpyrifos being the most frequently detected compound, followed by fipronil and atrazine. Higher concentrations were observed during the dry season, highlighting the influence of seasonality on
9
contamination. It is concluded that, despite the expansion of aquaculture, there are still shortcomings in controlling pesticide contamination, mainly associated with the intensive use of agrochemicals in areas surrounding aquaculture systems. Therefore, the study reinforces the urgent need to include AMPA in environmental monitoring parameters, as well as to adopt stricter policies for pesticide use and to promote good agricultural and aquaculture practices that ensure food safety and the protection of aquatic ecosystems.

COMMITTEE MEMBERS:
Interna - 1212049 - SYLVIANE BECK RIBEIRO
Interno - 1846780 - SANDRO DE VARGAS SCHONS
Externo à Instituição - RICARDO YSAAC GARCÍA NUÑEZ
Externo à Instituição - JERONIMO VIEIRA DANTAS FILHO