Banca de DEFESA: ANA CAROLINA ANCHIETA ADRIANO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA CAROLINA ANCHIETA ADRIANO
DATE: 13/06/2025
TIME: 09:00
LOCAL: Campus de Rolim de Moura- sala 21
TITLE:

Phytochemical and Toxicity Evaluation of Methanolic Extracts from the Leaves and Fruits (Pericarp) of Sapindus saponaria L. in Caenorhabditis elegans.

KEY WORDS:

Phytotoxicity; Herbal medicines; Longevity; Nematodes

PAGES: 57
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

Sapindus saponaria L. is recognized for containing triterpenoid saponins and glycosylated sesquiterpenes, bioactive compounds with antimicrobial and wound-healing properties, but with a toxicological potential that remains little explored. This study aimed to evaluate the toxicological and neurotoxic effects, as well as oxidative stress, of methanolic extracts from the leaves and fruit pericarp of the plant. Phytochemical analyses were performed using mass spectrometry (ESI-MS/MS) to characterize the compounds present, and bioassays were conducted using the experimental model Caenorhabditis elegans to assess toxicity through survival assays, lethal concentration, reproduction, body development, and lifespan. For the tests, methanolic extracts were prepared from leaves and fruits collected in the Western Amazon. The results indicated the presence of saponins and sesquiterpenes in the extracts, with greater diversity in the fruits. The bioassays revealed dose-dependent toxicity, with a median lethal concentration (LC₅₀) below 11 mg/mL. The leaf extract showed greater toxicity than the fruit extract, significantly affecting survival, body development, and reproduction, especially after 72 hours of exposure. However, the leaf extract prolonged the lifespan of C. elegans under certain experimental conditions. Statistical analyses confirmed the influence of dose, exposure time, larval stage, and extract type on the evaluated parameters. The findings reinforce the therapeutic potential of S. saponaria but also highlight the need to carefully assess the safety of its bioactive compounds. It is concluded that although the extracts exhibit promising pharmacological effects, standardization and dosage control are essential to prevent adverse effects, promoting the rational and safe use of the plant in health, antiparasitic, and cosmetic formulations.

COMMITTEE MEMBERS:
Interno - 1846780 - SANDRO DE VARGAS SCHONS
Externa à Instituição - PATRICIA PEREIRA - UFRGS
Externo à Instituição - PÉTERSON ALVES DOS SANTOS - UFRGS
Externo à Instituição - FRANCISCO CARLOS DA SILVA - São Lucas