Banca de DEFESA: JULIO CESAR CELESTINO FREITAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIO CESAR CELESTINO FREITAS
DATE: 31/03/2026
TIME: 08:30
LOCAL: https://meet.google.com/wbv-psfb-tba
TITLE:

Immunomodulatory activity of pariparoba (Pothomorphe umbellata) in tambaqui (Colossoma macropomum)

KEY WORDS:

Fish farming; medicinal plants, immunity, immunostimulants, herbal remedies.

PAGES: 63
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SUMMARY:

Aquaculture in Brazil has established itself as an important activity within Brazilian agribusiness, and in 2026, national production of farmed fish reached 1,011,540 tons, with tambaqui (Colossoma macropomum) standing out as the main native species produced in the country, accounting for 257,070 tons, placing the state of Rondônia in a prominent position as the leading producer of the species, responsible for 55,880 tons. However, aquatic health remains an obstacle to production, with various substances being used off-label to control microorganisms, negatively impacting the environment where they are produced and, consequently, posing risks to human health. Due to this, in search of alternatives, the use of immunomodulators in aquaculture has been studied. Among the plants with this potential is pariparoba (Pothomorphe umbellata), widely used in traditional medicine and with immunostimulant potential. Due to the need for alternatives to the use of chemotherapeutics in fish farming and the potential of pariparoba, the present study sought to evaluate the immunomodulatory activity through the supplementation of pariparoba leaves in tambaqui, assessing hematology, histopathology, and immunohistochemistry in acute inflammation. For this, four concentrations of supplementation using the plant's leaves were tested, added to commercial feed, and provided daily to the animals for thirty days. After this period, the groups were challenged, and blood and liver, spleen, and kidney tissue samples were collected at 12, 24, and 48 hours. Regarding hematimetric indices, there was no significant change in RBC, Ht, MCV, MCHC, and MCH at any of the concentrations tested. In leukocyte activity at 1% supplementation, a lower count was observed at the evaluated times, suggesting an anti-inflammatory effect. In groups 2, 3, and 4, no difference was observed compared to the positive control. In groups 2, 3, and 4, no difference was observed in relation to the positive control (p<0.05). In the analysis of phagocytes, after 48 hours, a reduction in activity was observed in all treatments analyzed, with emphasis on treatments 1, 2, and 3%, suggesting that lower doses of supplementation present better immunomodulation. In the histopathological analysis of the spleen, liver, and posterior kidney, no alterations were observed that compromised organ function. The percentage of immunolabeling of the cytokine IL-10 showed a response dependent on time and supplementation percentage, with greater immunolabeling observed after 24 and 48 hours of inflammatory induction. In iNOS, the liver was the organ that showed the highest labeling at all times (p<0.05), followed by the kidney and spleen. The results indicate that supplementation with pariparoba leaves at the tested concentrations was able to alter leukocyte expression, phagocytic activity, and IL-10 and iNOS expression, without causing changes in hematimetric, erythrocyte, and histological indices. Supplementation with 1 to 2% of pariparoba leaves for thirty days proved to be more effective in regulating the induced inflammatory process, without deleterious tissue alteration.

COMMITTEE MEMBERS:
Presidente - 1523620 - RUBIANI DE CASSIA PAGOTTO
Externa ao Programa - 2421710 - MAYRA ARAGUAIA PEREIRA FIGUEIREDO - UNIRExterno à Instituição - JOSEPH ALBERT MEDEIROS EVARISTO - FIOCRUZ