Banca de QUALIFICAÇÃO: PAULA DE CAMARGO MORATO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : PAULA DE CAMARGO MORATO
DATE: 21/06/2023
TIME: 09:00
LOCAL: virtual através do Google Meet meet.google.com/ivt-amjt-vyv
TITLE:

Viral diversity in two species of callitrichid primates from the southwestern Amazon in the context of One Health

KEY WORDS:

Weddell's Saddle-Black Tamarin. Rondon's Marmoset. Virome. Sentinel Species. Rondônia.

PAGES: 125
BIG AREA: Ciências Biológicas
AREA: Ecologia
SUBÁREA: Ecologia Aplicada
SUMMARY:

The loss of ecosystems, associated with climate change and the great potential for contact between humans, domestic and wild animals in areas of contact with tropical forests, leads to the overflow of microorganisms that can result in viruses and other epizootics. Non-human primates (NHPs), as they are the most susceptible to diseases that affect humans due to their evolutionary proximity, become essential models for disease studies and can be considered sentinels for epidemiological surveillance of many zoonotic diseases. The PNHs - mainly the small ones - are inserted in forest fragments impacted by increasing urbanization. In this context, to know the diversity of viral families presented in wild animals, especially in anthropized regions, leads us to early surveillance, because we know that most zoonoses are of epidemiological importance for human and animal health, thus contributing to the public health, conservation of species and the environment. The objectives of this work were to survey the viral families of the PNHs captured in two anthropized areas of Porto Velho/Rondônia, and to relate the composition of the virome with variables indicative of environmental quality. PNHs of two species of the Callitrichidae family - Mico rondoni (Rondônia marmoset) and Leontocebus weddelli (sauim) - were captured using baited traps of the live-trap type, Tomahawk model of two sizes, suspended on platforms, in urban forest fragments and periurban residents of the city of Porto Velho/RO. The two areas are made up of remnants of Open Ombrophylous Forest: the urban perimeter (Vila Tupi) has 21ha and the periurban perimeter (Universidade Federal de Rondônia - UNIR) has 55ha. Non-invasive collection of biological material (rectal swab) was carried out from 24 PNHs for the virome survey. The individuals' rectal swabs were grouped by species by location (species x location), giving us a total of four groupings called "pools", followed by massive sequencing (HTS) and bioinformatics analysis with the Kraken software for the characterization of diversity viral families, with emphasis on the families of viruses of health importance for mammals, including man. Thirty viral families were detected, of which 13 families have viruses of health importance for mammals. The UNIR area showed a greater richness of viral families (n=25), and both M. rondoni and L. weddelli had a greater viral richness, with 19 and ten families, respectively. The urban area (Tupi) had only 13 families, despite showing greater proximity between humans, PNHs and domestic animals. The UNIR area is located 1km from the open landfill, leading to a discussion about its relationship with the virome composition. Vegetation macrostructure (tree height, trees with CAP>20cm, canopy opening, distance from nearest neighbor) did not differ significantly between areas. The PNHs studied showed a richness of virome and may harbor a variety of families with viruses of health importance, showing the need for further research into these viral families to know their pathogenic importance for animals and the possible impact on public health and the species conservation

COMMITTEE MEMBERS:
Presidente - 2282448 - MARILUCE REZENDE MESSIAS
Interno - 1798567 - PAULO VILELA CRUZ
Externo à Instituição - LILIAN CATENACCI - UFPI