Banca de QUALIFICAÇÃO: LORAINNY CAROLINA LUCENA DE SOUZA

CYTOTOXIC, GENOTOXIC AND MUTAGENIC EVALUATION OF WATER FROM STREAMWAYS IN THE CITY OF PORTO VELHO – RO IN BIOASSAYS WITH ONION (Allium cepa)

Environmental mutagenicit; Cytogenetics; Water resources; micronucleus test.

Porto Velho (RO) has several streams that run through the city and, given urban growth and lack of basic sanitation, waste ends up being disposed of incorrectly, contaminating these water bodies. These streams are very close to industries, businesses and homes, increasing the likelihood of contamination. However, if there is contamination in the city's water supply, is it possible that these chemical elements in the water could induce chromosomal alterations? Is it possible to reach the genetic material of the organisms that are in direct or indirect contact with this water? Faced with this problem, the objective of this work is to evaluate the cytotoxic, genotoxic and mutagenic potential of water from streams in the municipality of Porto Velho-RO in bioassays with white onion (Allium cepa). At the time of collection, physical-chemical tests such as pH, oxygenation and water temperature were performed to characterize the water parameters. Collections were carried out in 5 streams, in two time periods: summer and winter. For each collection, 30 bulbs of white onion (Allium cepa) were cultivated. As a positive control group, paracetamol (800 mg/l) diluted in distilled water was used and, as a negative control, distilled water. In the present work, data referring to only 2 igarapés are presented, as a partial result. In total, 90 slides were produced for observation of 1000 cells per slide or 3000 (per bulb). The mitotic index, abnormalities in the mitotic cycle, such as the appearance of chromosomal bridges, presence of chromosomes or fragments, losses and micronucleus were analyzed. , As a result, it was possible to observe that Igarapé UNIR (winter and summer) presented toxicity and genotoxicity effect and Igarapé Viena (summer) presented genotoxic effect, but not mutagenic.