Banca de DEFESA: NEILSON DA SILVA COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : NEILSON DA SILVA COSTA
DATE: 16/10/2023
TIME: 14:00
LOCAL: Google Meet
TITLE:

ANATOMIC AND HISTOCHEMICAL CHARACTERIZATION OF Mabea dalyana Bigio et al

KEY WORDS:

Angiosperm; Anatomy; Euphorbiaceae, Cerrado, Amazon

PAGES: 29
BIG AREA: Ciências Biológicas
AREA: Ecologia
SUBÁREA: Ecologia de Ecossistemas
SUMMARY:

Mabea dalyana is a subshrub of the Euphorbiaceae family, endemic to the cerrados of Campos Amazônicos National Park and adjacent indigenous areas. The Genus Mabea comprises an average of 40 species distributed in countries of the Neotropical region, with a greater representation in the Amazon region. The objective of this research was to analyze and describe the anatomy of the vegetative and reproductive parts of Mabea dalyana. The materials were collected in Campos Amazônicos National Park and later taken to the Laboratory of Plant Physiology and Germplasm, Porto Velho, RO, Brazil. For the anatomical analyses, the samples were prepared following two procedures: anatomical characterization and histochemical characterization. The material was sectioned in the median region of the leaf freehand using disposable steel blades, the material was fixed in 70% FAA for 48 hours in refrigerator. Subsequently, the samples were dehydrated in increasing ethyl series (70%, 80%, 90% to absolute ethyl alcohol, for 2h each) and embedded in paraffin. After embedding, the material was sectioned on a rotary microtome with a thickness of 10 μm. For anatomical characterization, toluidine blue staining was performed. The images were obtained by photomicroscope and analyzed qualitatively as to their morphological aspect. For histochemical analysis, the sections were stained to identify the main classes of metabolites and their location in the vegetative organ. The slides were prepared in Canada Balsam and coverslip, examined and photographed under a photomicroscope). Subsequently, the slides were qualitatively analyzed for the presence and absence of secondary metabolite classes. In the leaf diaphanization process, about 2cm were submitted to bleaching, safranin staining (1% in ethanol 50%) and permanent slide mounting. The epidermis is unistratified on both sides. Cells with square and/or rectangular shape and straight walls. Heterogeneous chlorophyll parenchyma of dorsiventral type, containing one layer of palisade parenchyma and about 6 layers of spongy parenchyma cells. Central vein with open arched collateral type vascular bundle. Filling parenchyma with presence of idioblasts containing drusen. On the adaxial side, presence of a narrow layer of angular collenchyma. On both sides of the central vein it is possible to observe a thick cuticle. The secondary venation presents a bronchidrhodome pattern. The leaf is hypoestomatic, with paracitic stomas. Common epidermal cells with sinuous wall in both faces. Leaf type glabrous. Through histochemical analysis we identified the presence of cuticle and phenolic compounds in the leaf epidermis, as well as the presence of neutral polysaccharides and absence of starch, proteins and mucilages.

COMMITTEE MEMBERS:
Presidente - 1892574 - JAQUELINE MARTINS VASCONCELOS
Interna - 2146068 - MARCELA CAMPANHARO
Externo à Instituição - SEBASTIAO CARVALHO VASCONCELOS FILHO