Banca de QUALIFICAÇÃO: JULIO CESAR CELESTINO FREITAS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIO CESAR CELESTINO FREITAS
DATE: 17/12/2025
TIME: 16:00
LOCAL: https://meet.google.com/wbv-psfb-tba
TITLE:

Immunomodulatory activity of pariparóba (Pothomorphe umbellata) in tambaqui (Colossoma macropomum)

KEY WORDS:

Fish farming; medicinal plants, immunity, immunostimulants, herbal remedies.

PAGES: 45
BIG AREA: Ciências Biológicas
AREA: Imunologia
SUMMARY:

This study evaluated the immunomodulatory activity of dietary supplementation with pariparoba leaves (Photomorphe umbellata) in juvenile tambaqui (Colossoma macropomum), added to commercial feed, through hematological, biochemical, histopathological, and immunohistochemical tissue analyses of the supplemented animals and their controls. Ninety juvenile tambaqui (80±20g) were used, distributed in a completely randomized experimental design into 6 treatments, in 310L water tanks, with 15 fish per tank and designated as positive control, negative control, and levels of inclusion of pariparoba leaf – 10, 20, 30 and 40g/kg of leaves added to commercial feed, fed for 30 days, followed by 12, 24 and 48 hours of immunological challenge using 3x109 CFU of inactivated Aeromonas hydrophila, carried in 1 ml of phosphate buffer solution (PBS 7.2pH), injected into the coelomic cavity of all fish in the treatments, except the negative control. Hematologically, the hematocrit did not vary between treatments, but showed distinct temporal behavior within some groups. Red blood cell count decreased in T1 after 24h, suggesting a late onset of erythroid changes. MCV, MCHC, and MCH remained stable. T4 showed increased phagocytic respiratory activity at 24h, indicating dose-dependent oxidative stimulation; after 48h, T1 and T3 exhibited a decrease in this activity. All supplemented groups showed a sustained reduction in platelets from 12h onwards, suggesting modulation of cell release after inflammatory stimulation. Supplementation also altered total leukocytes, with an overall reduction after 48h. Monocytes decreased early in treatments T1 and T3, suggesting an anti-inflammatory effect, while T4 promoted an increase at 24h, indicating greater cell recruitment. After 48h, only T1 maintained significant monocytic suppression. Lymphocytes showed a reduction in all treatments, reinforcing the modulating action of pariparoba on this cellular compartment. Supplementation with pariparoba leaves modulated different components of the tambaqui's immune response in a dose- and time-dependent manner, mainly influencing monocytes, lymphocytes, thrombocytes, and phagocytic activity. The observed responses indicate the potential of Photomorphe umbellata as a phytogenic additive capable of regulating inflammatory processes and contributing to the immunocompetence of the species.

COMMITTEE MEMBERS:
Presidente - 1523620 - RUBIANI DE CASSIA PAGOTTO
Externa ao Programa - 2421710 - MAYRA ARAGUAIA PEREIRA FIGUEIREDO - UNIRExterna ao Programa - 1554302 - ELIETH AFONSO DE MESQUITA - UNIR