Banca de QUALIFICAÇÃO: LARISSA SIMÔNI DOMINGOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LARISSA SIMÔNI DOMINGOS
DATE: 17/12/2025
TIME: 14:00
LOCAL: https://meet.google.com/wbv-psfb-tba
TITLE:

Histopathological and immunohistochemical evaluation of the inflammatory response of cat's tail (Acalypha arvensis) in tambaqui (Colossoma macropomum) challenged by Aeromonas hydrophila.

KEY WORDS:

Fish farming; medicinal plants; iNOS CD4⁺; fish immunity.

PAGES: 43
BIG AREA: Ciências Biológicas
AREA: Imunologia
SUMMARY:

Acalypha arvensis is a plant widely used in traditional medicine and with immunomodulatory potential for application in aquaculture. In tambaqui (Colossoma macropomum), the use of phytogenic additives can strengthen the immune response and reduce damage associated with bacterial infections, such as those caused by Aeromonas hydrophila. Thus, this study evaluated the immunomodulatory effects of dietary supplementation with A. arvensis leaves on histopathological and immunohistochemical parameters (iNOS and CD4⁺) in liver, spleen, and kidney after experimental challenge. One hundred and eight tambaqui were used, distributed into six treatments: negative control (C–), positive control (C⁺), and four groups supplemented with A. arvensis (T1–T4: 10, 20, 30, and 40 g/kg). The fish were fed for 30 days and subsequently challenged with inactivated A. hydrophila (except C–). Liver, spleen, and kidney samples were collected at 12, 24, and 48 hours post-challenge. Histopathological analysis was performed using HE staining and immunohistochemistry for iNOS (innate immunity) and CD4⁺ (adaptive immunity). Quantification was conducted using QuPath, and the normality of residuals and confirmatory statistics were evaluated using GraphPad Prism. Supplementation with A. arvensis promoted dose- and time-dependent immunomodulation. iNOS showed initial peaks (12 hours), indicating early inflammatory activation; T1 and T2 exhibited a more controlled response, while T3 and T4 showed sustained activation without inducing significant lesions. CD4⁺ progressively increased from 24 hours onwards, with higher expression in T3 and T4, evidencing efficient adaptive activation. Histopathology identified lower lesion intensity in the supplemented groups compared to the C⁺ group, which presented congestion, splenic hyperplasia, and renal tubular degeneration. T1 and T2 doses better preserved tissue architecture, while higher doses promoted a robust response without toxicity. No supplemental treatment resulted in necrosis or irreversible damage.

COMMITTEE MEMBERS:
Presidente - 1523620 - RUBIANI DE CASSIA PAGOTTO
Externa ao Programa - 1554302 - ELIETH AFONSO DE MESQUITA - UNIRExterno à Instituição - JEFFERSON YUNIS AGUINAGA